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Proteinase K Solution (20 mg/ml)

Cat No. Concentration Price (EGP)
BT03AA 20 mg/ml

Description

Proteinase K is a nonspecific serine protease that is used for general digestion of proteins produced by Engyodontium album fungus.

Proteinase K remains active:

  • Over a wide pH range—optimal activity between 6.5 and 9.5
  • Under denaturing conditions (e.g., in the presence of SDS or urea)
  • In the presence of metal chelating agents (e.g., EDTA)
  • At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications

Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.